Concentration change of catecholamine and leucine - enkephalin in sheep cerebrospinal fluid after apa microencapsulated bovine chromaffin cells transplantation to the spinal subarachnoid space 微囊化牛嗜鉻細(xì)胞腦脊液亮氨酸腦啡肽和兒茶酚胺含量的變化
Collection and preservation of samples : as soon as the three vital signs disappeared , the dogs were anatomized , and the heart , liver , kidney , spleen , lung , brain , muscle in the injection location and no injection location , the heart blood , urine , bile , cerebrospinal fluid ( csf ) in the lateral ventricle and spinal subarachnoid space , spinal cord ( medulla oblongata , cervical cord , the upper beast spinal cord , breast spinal cord and waist spinal cord ) were taken out , some of which were preserved at - 20 for qualitative and quantitative analysis , and the others were fixed with 4 % formaldehyde for the pathology observation 3 、樣品采集:當(dāng)心電、血壓和呼吸全部消失時(shí),迅速解剖動(dòng)物,采取心臟、肝臟、腎臟、脾臟、肺臟、大腦、注射部位肌肉、注射部位20cm以外肌肉、心血、尿液、膽汁、側(cè)山西醫(yī)科大學(xué)碩士學(xué)位論文腦室腦脊液、脊髓腔腦脊液和不同節(jié)段的脊髓(包括延髓、頸髓、上胸部脊髓、胸部脊髓和腰部脊髓)等組織,冷凍保存。 4 、病理觀察:采取心臟、肝臟、 ’腎臟、脾臟、肺臟、大腦、脊髓等組織, 4 %甲醛固定,石蠟包埋,切片, he染色,光鏡觀察。